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mouse monoclonal mcp 1 antibody  (R&D Systems)


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    Structured Review

    R&D Systems mouse monoclonal mcp 1 antibody
    Mouse Monoclonal Mcp 1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+ccl2+antibody/Human+CCL2%2FJE%2FMCP-1+Antibody/pm41964752-35-10-15
    Average 94 stars, based on 61 article reviews
    mouse monoclonal mcp 1 antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Control:

    Article Title: ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NFκB activation
    Article Snippet: Bay11-7082 (S2913) was purchased from Selleck Chemicals (Houston, TX, USA). .. Antibodies against the following proteins were used in this study: Padi2 (66386–1-Ig; Proteintech, Rosemont, IL, USA), α-tubulin (sc-8035; Santa Cruz Biotechnology, Inc., Dallas, Texas, USA), β-actin (sc-47778; Santa Cruz Biotechnology.), LaminA/C (sc-376248; Santa Cruz Biotechnology), p21 (#2947; Cell Signaling Technology, Inc., Danvers, MA, USA), mouse CCL2 antibody (AB-479-NA; R&D Systems, Inc, Minneapolis, MN, USA), mouse CCL5 antibody (AF478; R&D Systems), mouse CCL7 antibody (AF-456-NA; R&D Systems), and normal goat IgG control (AB-108-C; R&D Systems). .. The following siRNAs used in the present study were purchased from Origene (Rockville, MD, USA): Padi2 siRNA (SR418983B and C), RelA siRNA (SR427138A), Ccl2 siRNA (SR403037C), Ccl5 siRNA (SR400775A), Ccl7 siRNA (SR400957C), and negative control siRNA (SR30004).

    Article Title: ROS-induced PADI2 downregulation accelerates cellular senescence via the stimulation of SASP production and NFκB activation.
    Article Snippet: Bay11-7082 (S2913) was purchased from Selleck Chemicals (Houston, TX, USA). .. Antibodies against the following proteins were used in this study: Padi2 (66386–1-Ig; Proteintech, Rosemont, IL, USA), α-tubulin (sc-8035; Santa Cruz Biotechnology, Inc., Dallas, Texas, USA), β-actin (sc-47778; Santa Cruz Biotechnology.), LaminA/C (sc-376248; Santa Cruz Biotechnology), p21 (#2947; Cell Signaling Technology, Inc., Danvers, MA, USA), mouse CCL2 antibody (AB-479-NA; R&D Systems, Inc, Minneapolis, MN, USA), mouse CCL5 antibody (AF478; R&D Systems), mouse CCL7 antibody ROS‐induced PADI2 downregulation accelerates cellular senescence via the stimulation of... 1 3 Page 3 of 20 155 (AF-456-NA; R&D Systems), and normal goat IgG control (AB-108-C; R&D Systems). .. The following siRNAs used in the present study were purchased from Origene (Rockville, MD, USA): Padi2 siRNA (SR418983B and C), RelA siRNA (SR427138A), Ccl2 siRNA (SR403037C), Ccl5 siRNA (SR400775A), Ccl7 siRNA (SR400957C), and negative control siRNA (SR30004).

    Membrane:

    Article Title: CCL2-mediated endothelial injury drives cardiac dysfunction in long COVID
    Article Snippet: Proteins were separated by electrophoresis and transferred to mini nitrocellulose membranes (Bio-Rad) using a semi-dry-based transfer system (Bio-Rad). .. The membrane was blocked in 5% BSA and incubated overnight with the following antibody dilutions: human CCL2 antibody (R&D systems, AF-279-NA, 1:1000), mouse CCL2 antibody (R&D systems, AF-479-NA, 1:1000), human or mouse pRyR2 antibody (Thermo Fisher Scientific, PA5-36758, 1:500), RyR2 antibody (Abcam, ab196355, 1:500), followed by incubation for 1 h with HRP-conjugated secondary antibody. .. The housekeeping protein ß-actin was blotted by incubating with ß-actin control antibody (R&D systems, MAB8929-SP, 1:2000).

    Incubation:

    Article Title: CCL2-mediated endothelial injury drives cardiac dysfunction in long COVID
    Article Snippet: Proteins were separated by electrophoresis and transferred to mini nitrocellulose membranes (Bio-Rad) using a semi-dry-based transfer system (Bio-Rad). .. The membrane was blocked in 5% BSA and incubated overnight with the following antibody dilutions: human CCL2 antibody (R&D systems, AF-279-NA, 1:1000), mouse CCL2 antibody (R&D systems, AF-479-NA, 1:1000), human or mouse pRyR2 antibody (Thermo Fisher Scientific, PA5-36758, 1:500), RyR2 antibody (Abcam, ab196355, 1:500), followed by incubation for 1 h with HRP-conjugated secondary antibody. .. The housekeeping protein ß-actin was blotted by incubating with ß-actin control antibody (R&D systems, MAB8929-SP, 1:2000).



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